The course consists of a lecture and excercise to deepen knowledge in several neurohistological methods in mammals. Topics include stereotaxis, fixation and preparation of nervous tissue, sectioning, immunostaining, tracing methods, analysing of stained sections with bright field, epifluorescence and confocal laser scanning microscopy. Furthermore, methods for preparing high quality images from multi-immunostainings are applied to prepared sections. Finally, the students will analyse and discuss their results in respect to related publications and prepare a presentation. The students work in groups of 2-3 on individual projects and present the outcome within the class.
- Teacher: Alexander Kaiser